THE EFFECT OF LEUKOCYTOSPERMIA ON SPERM QUALITY IN INFERTILE MEN VISITING NATIONAL IN-VITRO FERTILIZATION CENTER–MISURATA
Keywords:
Leukocytospermia, Semen specimens, MGT, Spermatogenesis, testis, LymphocytesAbstract
Leukocytospermia is highly prevalence condition among infertile males. However, the role of Leukocytospermia in the pathogenesis of male infertility remains controversial. Our study aimed to evaluate the prevalence of Leukocytospermia in study population and assessment the effect of seminal leukocytes on various semen parameters.This is a prospective study conducted between June-October 2017 at National Infertility Center/ Misurata-Libya. 187 infertile men with no history of azoospermia, varicocele and any chronic underling diseases like diabetic, hypertensive and chronic viral infection were included. The demographic data that include age, infertility type, infertility period, and smoking status were collected from their medical records. Semen specimens were examined for ejaculation volume, acidity degree (pH), sperm concentration, sperm motility percent, sperm progressive motility percent, sperm morphology percent and leukocytes concentration according to the standard method of the WHO 2010 guidelines. Therefore, participants were grouped based on seminal leukocytes concentration into (Group A :< 2 WBC/HPF; Group B: 2-4 WBC/HPF; Group C: 4-6 WBC/HPF; Group D: <6WBC/HPF).All collected data were analyzed by Statistical Package for the Social Sciences (SPSS) version 20 using chi-squared X2 test, one way analysis of variance (ANOVA) and Fisher,s least significant difference LSD test. A P value ≤ 0.05 was defined as statistically significant.The study found that, 42 (34.8%) of study population have Leukocytospermia (≥3WBC/HPF). The sperm parameters, which have effected significantly by increase of seminal leukocytes were total motility (F 5.381; P 0.001<0.05), progressive motility (F 3.022; P 0.031<0.05) and normal morphology (F 3.518; P 0.02<0.05). The total motility was significantly higher in Group A (62.69±17.63; P<0.05) compare with other Groups, and was significantly lower in Group D (43.21±26.25; P<0.05) especially compare with Group A. The progressive motility was a significantly lower in Group D (11.57±7.6; P<0.05) compare to rest of leukocytes concentration Groups. The normal morphology was significantly higher in Group A (62.69±17.63; P<0.05) compare with Group B (7.92±8.22; P<0.01) and C (8.13±7.30; P<0.05), while, it was not any difference between Group D (10.11±11.03; P> 0.05) and other Groups.The study concluded that seminal leukocytes have bi-effects of sperm quality. While, they caused of deterioration of different motility forms of sperm, they contributed of improvement of normal morphology percentage especially in high concentration level. However, Future studies are needed to examine bi-effects of seminal leukocytes on sperm quality
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