ISOLATION AND IDENTIFICATION OF SOME PATHOGENIC FUNGI ASSOCIATED WITH STORED BARLEY GRAINS IN ZLITEN AREA, LIBYA
Keywords:
.Abstract
The deterioration of barley grains during their storage by fungi has been a problem of economic importance, especially in the use of grains barley for soowinging and other uses. Results of the present study could be summarized in the following points, the isolated fungi were differed considerably in their number and kinds according to the inspected locality. Generally, the isolated fungi from stored barley grains were represent 10 species belonging to 6 genera. These fungi were purified and identified as Aspergillusflavus, Aspergillusochraceus, Aspergillussydowii, Chaetomiumglobosum, Cladosporiumcladosporioides, Eurotiumarnstelodami, Eurotiumintermedium, E. repens, Penicilliumchrysogenum, and Rhizopusstolonifer. The most frequent fungi were Aspergillusflavus and Rhizopusstolonifer. On the other hand, Cladosporiumcladosporioides, Eurotiumintermedium, Chaetomiumglobosum and Eurotiumarnstelodami were less frequency. The pathogenicity test using healthy barley grains and 5 different of isolated fungi showed that Aspergillusflavus, followed by Aspergillusochraceus and Rhizopusstolonifer were significantly more pathogenic compared with the control as they decrease grain germination percentage to 33.3% for each one and they caused 100.0 , 93.3 and 93.3% of kernel invasion, respectively. On the contrary, Aspergillussydowii and Penicilliumchrysogenum were the least pathogenic. The analogous values for grain invasion and germinability were 100.0 and 60.0% for each one. However, the difference between any treatment and the control was significant.
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