SEEDS AND THEIR BIO-CONTROL IN VITRO

المؤلفون

  • Salem Milad AL-Asmarya Islamic University, Faculty of Science, Department of Microbiology, Libya
  • Ali Ben Milad AL-Asmarya Islamic University, Faculty of Science, Department of Microbiology, Libya

DOI:

https://doi.org/10.59743/jbs.v30i.78

الملخص

Various fungal species (Eleven species) belonging to different five genera have been.  ‘‘Malty and Blanka’’  found in or on localized broad bean seeds cultivar. These fungi were purified and identified as Aspergillus flavipes, Aspergillus niger, Aspergillus terreus, Aspergillus ustus, Aspergillus wentii, Penicillium brevicompactum, Penicillium expansum, Fusarium moniliforme, Rhizopus stolonifer and Mucor eircinellricles.                     

Results also indicated that Aspergillus flavus isolate No. (1) was significantly more pathogenic as it totally inhibited seed germination (0%) and caused the highest percentage of seed infection (100%), followed by Aspergillus niger isolate No.(1) and Aspergillus flavus isolate No. (3). It was found that Aspergillus flavus isolate No. (2) and No (5) were the least pathogenic. However, the difference between any two tested fungi was significant. In this study showed that Trichoderma album and Trichoderma  harzianum significantly inhibited the growth of Penicillium expansum by 77.7 and 72.2%, respectively, followed by Aspergillus flavus and Aspergillus flavipes. However, Aspergillus ustus was the least affected fungus in this concern (22.2%).

المراجع

Abdel-Hafes , A.I.I.(1988).Mycoflora of broad bean , chickpea and lentil seeds in Egypt. Cryptogamine Mycologie, 9:335-363.

Abdul Wehab S.A, S.A, El-Nagerabi and A. El-shafie, (2015), Leguminicolous fungi associated with some seeds of Sudanese legumes, Biodiversitas 16,No.2 269-280.

Anonymous(1959). International Rules For Seed Testing. Int. Seed Testing Assoc. Proc. 24:275-584.

Barnett, H.I. and B.B. Hunter (1972). Illustrated Genera of Imperfect Fungi. Burguess, Minneapolis.

Bean,G . and T. Fernando (1985). Winged bean (Psoohocapus tetragonolobus L.) as a substrate for growth and aflatoxin production by aflatoxigenic strains of Aspergillus spp. Mycopathologia 90:141-145.

Bhat, R.V .and S. Vasanthi(2003). Mycotoxin food safety risks in developing countries . Food Safety in food Trade . Vision 2020 for food, Agriculture and Environment , focus 10:1-2.

Chat, I. Y. Henis and R. Mitchell (1967). Chemical composition of hyphal and sclerotial walls of Sclerotium rolfsii Sacc. Can.J. Microbiol., 13:137-141.

Christensen, C. M. and R. F. Drechslera (1954). Grain storage studies. 14. Changes in moisture content, germination percentage and moldness of wheat samples stored in different portions of bulk wheat commercial bines. Cereal chem., 31:206-216.

El-Sayed, F. H. Nakoul and P. Williams (1982). Distribution of protein content in the world collection of faba bean (Vicia faba L.) FABIS, 5:37.

El-Wakil, M. A, I. M. El-Refai O.A. Awadallah, M. A. El-Metwally and M. S. Mohammed (2009). Seed- borne pathogens of faba bean in Egypt : Detection and Pathogenicity. Plant Pathol. J., 8:90-97.

Fahmy, T.A. (1960). Fumigation of rice against insect and its effect on the quality of the grain. M.Sc. Thesis, Cairo University.

Fisher , R. A. (1984). Statistical Methods 6th ed. Iowa State Univ. press, Ames, Iowa, USA.

Hansen, H. N. (1926). A simple method of obtaining single spore culture. Science, 64 :384, 1659.

ISTA (International Seed Testing Association) (1966). International Rules Health Testing Proc. International Seed Test Assoc. 31, 1-152.

Rao, V. V. R., M. S. Reddy and K. C. Roa (1996). Effect of fungicidal treatment on the viability of groundnut seeds in storage. Seed Res., 24:66-68.

Raper, K. B. and D. I. Fennel (1965). The Genus Aspergillus. Williams and Wilkins Company, Baltimore, 686 pp.

Riker, A. J. and R. S. Riker (1936). International to Research on Plant Diseases. John. S. Swift Co. Inc. Sta. Iovis, Chicago, New York.

Saber, S. M. (1992). Fungal contamination, natural occurrence of mycotoxins and resistance for aflatoxin accumulation of some broad bean (Viba faba L.) cultivar. J. Basic Microbiol 32 (4) : 249-258.

Simon, C. and M. Sivasithamparam (1988). Interactions among G. graminis var. tritici, Trichoderma kooningii and soil bacteria. Can. J. microbial., 34:871-876.

Snedecor, G. W. and SK. Alane (2013). Efficacy of some botanical against seed borne Fungi or green gram (Phaseolus aureus Robb.) Biosci Discov., 4(1), 107-110.

Tseng, T. C. J. C. Tu and the L. C. Soo (1995). Comparison of the profiles of seeds borne fungi and the occurrence of aflatoxins in mould-damaged bean and soybeans. Microbiol., 84:105-116.

Zhou. T. and R. D. Releder (1990). Selection of Epicoccum purpurascens and improved biocontrol of Sclerotinia sclerotiorum. Can . J. Microbial., 36:754-759.

التنزيلات

منشور

2017-06-30

إصدار

القسم

مقالات

كيفية الاقتباس

SEEDS AND THEIR BIO-CONTROL IN VITRO (S. Milad & A. Ben Milad). (2017). مجلة العلوم الأساسية, 30, 38-54. https://doi.org/10.59743/jbs.v30i.78