STUDIES ON TOMATO WILT DISEASE IN ZLITEN CITY LIBYA
Abstract
In the present investigation, certain aspects of the tomato wilt disease caused by Fusarium oxysporum f.sp. lycopersici were studied. The obtained results could be summarized as follows: Disease survey revealed that tomato wilt disease was spread in all inspected localitiesat Libya (Adwawo, Azdo, El-Dafnia, Keam and Saratan). Isolation trials yieded sixty different fungal isolates belonging to 6 genera and 6 species. The count of isolated fungi was differed from one locality to another. Samples obtained from Azdo locality showed the highest percentage of isolated fungi, while the lowest one was obtained from Adwawo locality. Among the isolated fungi, Fusarium oxysporum f.sp. lycopersici was the most prevalent fungus. Pathogenic capabilities of twelve isolates of the pathogen showed that isolates varied in their pathogenicity and isolate No.32, isolated from Azdo locality, was more pathogenic while isolate No. 13, isolated from Saratan locality was the least virulent. The other tested isolates had intermediate effect in this concern. Six isolates of a bioagent (Trichoderma) were screened in vitro to test their effect on the growth of more pathogenic isolates of the pathogen. Trichodermaharzianum isolate No.(1) was found to be the most potent antagonistic agent to all the tested pathogenic isolates of the pathogen without significant difference with T. harzianum isolate No.3 (which previousley isolated from tomato rhizosphere in Libya). On contrary, T. harzianum isolate No.4 and 2 were the least effective in this respect. The most tolerant pathogenic isolate against the effect of different Trichoderma species was isolate No.20, which isolated from El-Dafnia locality.
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