مقاومة المضادات الحيوية في عزلات بكتيرية من عينات المياه العذبة ومياه البحر في مدينة زليتن - ليبيا

سعاد شعبان محمد الدبرزي; منصور الحولي; القاسم شكشك; علي   المشغب; حنان  التونسي

doi:10.59743/jmset.v10i1.182

Authors

Soad Adbarzi قسم علم الحيوان، كلية العلوم، الجامعة الاسمرية الاسلامية، زليتن، ليبيا
Mansor M. Alholi المعهد العالي للعلوم والتقنية قصر الأخيار، قصر الأخيار، ليبيا
Alkasm H. Shukshuk قسم علم الحيوان، كلية العلوم، الجامعة الاسمرية الاسلامية، زليتن، ليبيا
Ali Almashgab قسم علم الحيوان، كلية العلوم، الجامعة الاسمرية الاسلامية، زليتن، ليبيا
Hanan O.M. Altunsi قسم علم الحيوان، كلية العلوم، الجامعة الاسمرية الاسلامية، زليتن، ليبيا

DOI:

https://doi.org/10.59743/jmset.v10i1.182

Keywords:

Antibiotic resistance bacteria, 16S rRNA gene sequencing, Mediterranean Sea, Wadi kaam water, Zliten Libya

Abstract

Water is the most favorable surrounding for various life forms to grow. The aquatic environments are recognized reservoirs of the broad spectrum of antibiotic resistance bacteria, including pathogenic ones, which have the potential to spread antibiotic resistance to different environments and pose risks to public and environmental health. This work estimated resistance of sixth identified bacterial isolates from sea and fresh water in Zliten, Libya to frequently prescribed five antibiotics. It was observed that the first sequence LC532105 from wadi kaam water shows similarity with Citrobacter freundii strain NCTC9750,Second sequence LC532106 shows similarity with Bacillus pumilus strain ATCC 7061, Third sequence LC532107 shows similarity with Bacillus megaterium strain ATCC 14581 , Fourth sequence from sea water shows similarity with Citrobacter freundii strain NCTC9750,fifth sequence shows similarity with Proteus vulgaris strain ATCC 29905 while last sequence shows similarity with Pseudomonas aeruginosa strain DSM 50071. First and fourth type strain (Citrobacter freundii) is not toxic and second, third, fifth and sixth types were toxic especially Pseudomonas aeruginosa. All bacterial isolates from both sampling sites showed resistance against cefotaxime. A continuous analysis for physicochemical and antibiotic resistance profile of bacterial isolates of surface water are frequently required.

Downloads

Download data is not yet available.

Author Biography

Soad Adbarzi, قسم علم الحيوان، كلية العلوم، الجامعة الاسمرية الاسلامية، زليتن، ليبيا

ا

References

Vilca, F. Z., & Angeles, W. G. (2018). Occurrence of Antibiotics Residues in the Marine Environment. Examines Mar. Biol. Ocean, 2, 12-14.‏

Irfan, S., & Alatawi, A. M. M. (2019). Aquatic ecosystem and biodiversity: a review. Open Journal of Ecology, 9(1), 1-13.‏

Hassan, B., Qadri, H., Ali, M. N., Khan, N. A., & Yatoo, A. M. (2020). Impact of climate change on freshwater ecosystem and its sustainable management. Fresh Water Pollution Dynamics and Remediation, 105-121.‏

World Health Organization (2017). WHO Publishes List of Bacteria for Which New Antibiotics Are Urgently Needed. World Health Organization [Internet]Available: https://www.who.int/news/item/27-02-2017-who-publishes-list-of-bacteria-for-which-new-antibiotics-are-urgently-needed (Accessed November 15, 2021).

World Health Organization (2021). Antimicrobial Resistance. World Health Organization [Internet]. 2021.

[Internet]Available: https://www.who.int/news-room/fact sheets/detail/antimicrobial-resistance (Accessed November 15, 2021).

Poretsky, R., Rodriguez-R, L. M., Luo, C., Tsementzi, D., & Konstantinidis, K. T. (2014). Strengths and limitations of 16S rRNA gene amplicon sequencing in revealing temporal microbial community dynamics. PloS one, 9(4), e93827.‏

Moyane, J. N., Jideani, A. I. O., & Aiyegoro, O. A. (2013). Antibiotics usage in food-producing animals in South Africa and impact on human: Antibiotic resistance. Afr. J. Microbiol. Res, 7(24), 2990-2997.‏

Batt, A. L., Kim, S., & Aga, D. S. (2007). Comparison of the occurrence of antibiotics in four full-scale wastewater treatment plants with varying designs and operations. Chemosphere, 68(3), 428-435.‏

Schwartz, T., Kohnen, W., Jansen, B., & Obst, U. (2003). Detection of antibiotic-resistant bacteria and their resistance genes in wastewater, surface water, and drinking water biofilms. FEMS microbiology ecology, 43(3), 325-335.‏

AbdelRahim, K. A. A., Hassanein, A. M., & Abd El, H. A. E. H. (2015). Prevalence, plasmids and antibiotic resistance correlation of enteric bacteria in different drinking water resources in sohag, egypt. Jundishapur journal of microbiology, 8(1).‏

Guzman-Otazo, J., Gonzales-Siles, L., Poma, V., Bengtsson-Palme, J., Thorell, K., Flach, C. F., ... & Sjöling, Å. (2019). Diarrheal bacterial pathogens and multi-resistant enterobacteria in the Choqueyapu River in La Paz, Bolivia. PLoS One, 14(1), e0210735.‏

Singh, A. K., Das, S., Kumar, S., Gajamer, V. R., Najar, I. N., Lepcha, Y. D., ... & Singh, S. (2020). Distribution of antibiotic-resistant Enterobacteriaceae pathogens in potable spring water of eastern Indian Himalayas: emphasis on virulence gene and antibiotic resistance genes in Escherichia coli. Frontiers in Microbiology, 11, 581072.‏

Guardabassi, L., Petersen, A., Olsen, J. E., & Dalsgaard, A. (1998). Antibiotic resistance in Acinetobacter spp. isolated from sewers receiving waste effluent from a hospital and a pharmaceutical plant. Applied and Environmental Microbiology, 64(9), 3499-3502.‏

Chagas, T. P. G., Seki, L. M., Cury, J. C., Oliveira, J. A. L., Dávila, A. M. R., Silva, D. M., & Asensi, M. D. (2011). Multiresistance, beta‐lactamase‐encoding genes and bacterial diversity in hospital wastewater in Rio de Janeiro, Brazil. Journal of applied microbiology, 111(3), 572-581.‏

Marathe, N. P., Shetty, S. A., Shouche, Y. S., & Larsson, D. J. (2016). Limited bacterial diversity within a treatment plant receiving antibiotic-containing waste from bulk drug production. PLoS One, 11(11), e0165914.‏

Turolla, A., Cattaneo, M., Marazzi, F., Mezzanotte, V., & Antonelli, M. (2018). Antibiotic resistant bacteria in urban sewage: Role of full-scale wastewater treatment plants on environmental spreading. Chemosphere, 191, 761-769.‏

Adbarzi, S. S. M., Tripathi, P., Choudhary, K. K., Kant, R., & Tripathi, V. (2020). Assessment of physico-chemical properties of pre and post-treated wastewater of Prayagraj region and its effect on nearby Ganges river. Vegetos, 33(2), 258-264.‏

Alexander, J., Hembach, N., & Schwartz, T. (2020). Evaluation of antibiotic resistance dissemination by wastewater treatment plant effluents with different catchment areas in Germany. Scientific Reports, 10(1), 8952.‏

Amarasiri, M., Sano, D., & Suzuki, S. (2020). Understanding human health risks caused by antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARG) in water environments: Current knowledge and questions to be answered. Critical Reviews in Environmental Science and Technology, 50(19), 2016-2059.‏

Chauhan, N. S., & Punia, A. (2023). Antibiotic pollution and antibiotic-resistant bacteria in water bodies. In Degradation of Antibiotics and Antibiotic-Resistant Bacteria from Various Sources (pp. 179-201). Academic Press.‏

Henriques, I. S., Fonseca, F., Alves, A., Saavedra, M. J., & Correia, A. (2006). Occurrence and diversity of integrons and β-lactamase genes among ampicillin-resistant isolates from estuarine waters. Research in microbiology, 157(10), 938-947.‏

Zhang, X. X., Zhang, T., & Fang, H. H. (2009). Antibiotic resistance genes in water environment. Applied microbiology and biotechnology, 82, 397-414.‏

Guo, X., Tang, N., Lei, H., Fang, Q., Liu, L., Zhou, Q., & Song, C. (2021). Metagenomic analysis of antibiotic resistance genes in untreated wastewater from three different hospitals. Frontiers in Microbiology, 12, 709051.‏

Berger-Bächi, B. (2002). Resistance mechanisms of gram-positive bacteria. International Journal of Medical Microbiology, 292(1), 27-35.‏

CytoGene Research & Development Laboratory : K-51, Agro Park, UPSIDC Industrial Area, Kursi Road (Lucknow) Dist-Barabanki – 2250.

Murray, P.R., Baron, E.J., Pfaller, M.A., Tenover, F.C. & Yolken, R.H.. (1995). Manual of Clinical Microbiology, 6th edition. American Society of Microbiology Press, Washington DC. 1482 p.

Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing: Twenty Sixth Informational Supplement. CLSI Document M100-S26”. Wayne, PA: Clinical and Laboratory Standards Institute (2016).

World Health Organization (2014). Antimicrobial Resistance Global Report on Surveillance. Geneva: World Health Organization. Available at: https://apps.who.int/iris/handle/10665/112642 (Accessed November 12, 2021).

World Health Organization (2015). Global Action Plan on Antimicrobial Resistance. Geneva: World Health Organization. Available at: https://www.who.int/publications/i/item/9789241509763. (Accessed November 15, 2021).

Adler, A., Katz, D. E., & Marchaim, D. (2016). The continuing plague of extended-spectrum β-lactamase–producing Enterobacteriaceae infections. Infectious Disease Clinics, 30(2), 347-375.‏

Bush, K. (2018). Past and present perspectives on β-lactamases. Antimicrobial agents and chemotherapy, 62(10), 10-1128.‏

Bush, K., & Bradford, P. A. (2020). Epidemiology of β-lactamase-producing pathogens. Clinical microbiology reviews, 33(2), 10-1128.‏

Tacão, M., Laço, J., Teixeira, P., & Henriques, I. (2022). CTX-M-producing bacteria isolated from a highly polluted river system in Portugal. International Journal of Environmental Research and Public Health, 19(19), 11858.‏

Yamaguchi, A. (1997). Bacterial resistance mechanisms for tetracyclines. Nihon rinsho. Japanese Journal of Clinical Medicine, 55(5), 1245-1251.‏

Nawaz, M., Khan, A. A., Khan, S., Sung, K., & Steele, R. (2008). Isolation and characterization of tetracycline-resistant Citrobacter spp. from catfish. Food microbiology, 25(1), 85-91.‏

Poirel, L., Ros, A., Carricajo, A., Berthelot, P., Pozzetto, B., Bernabeu, S., & Nordmann, P. (2011). Extremely drug-resistant Citrobacter freundii isolate producing NDM-1 and other carbapenemases identified in a patient returning from India. Antimicrobial agents and chemotherapy, 55(1), 447-448.‏

Raman, G., Avendano, E. E., Chan, J., Merchant, S., & Puzniak, L. (2018). Risk factors for hospitalized patients with resistant or multidrug-resistant Pseudomonas aeruginosa infections: a systematic review and meta-analysis. Antimicrobial Resistance & Infection Control, 7, 1-14.‏

Tabak, Y. P., Merchant, S., Ye, G., Vankeepuram, L., Gupta, V., Kurtz, S. G., & Puzniak, L. A. (2019). Incremental clinical and economic burden of suspected respiratory infections due to multi-drug-resistant Pseudomonas aeruginosa in the United States. Journal of Hospital Infection, 103(2), 134-141.‏

Kunz Coyne, A. J., El Ghali, A., Holger, D., Rebold, N., & Rybak, M. J. (2022). Therapeutic strategies for emerging multidrug-resistant Pseudomonas aeruginosa. Infectious diseases and therapy, 11(2), 661-682.‏

Livermore, D. M., Williams, J. D., & Davy, K. W. (1985). Cephalosporin resistance in Pseudomonas aeruginosa, with special reference to the proposed trapping of antibiotics by beta-lactamase. Chemioterapia: International Journal of the Mediterranean Society of Chemotherapy, 4(1), 28-35.‏

Kim, E. S., & Hooper, D. C. (2014). Clinical importance and epidemiology of quinolone resistance. Infection & chemotherapy, 46(4), 226-238.‏

Hooper, D. C., & Jacoby, G. A. (2015). Mechanisms of drug resistance: quinolone resistance. Annals of the New York academy of sciences, 1354(1), 12-31.‏

Adbarzi, S. S. M., Tripathi, P., Kant, R., & Tripathi, V. (2020). Assessment of bacterial diversity and their antibiotic resistance profiles in wastewater treatment plants and their receiving Ganges River in Prayagraj (Allahabad), India. Vegetos, 33(4), 744-749.‏

Berger-Bächi, B. (2002). Resistance mechanisms of gram-positive bacteria. International Journal of Medical Microbiology, 292(1), 27-35.‏

Munita, J. M., Bayer, A. S., & Arias, C. A. (2015). Evolving resistance among Gram-positive pathogens. Clinical Infectious Diseases, 61(suppl_2), S48-S57.‏

Duc, L. H., Hong, H. A., Barbosa, T. M., Henriques, A. O., & Cutting, S. M. (2004). Characterization of Bacillus probiotics available for human use. Applied and environmental microbiology, 70(4), 2161-2171.‏

Adamski, P., Byczkowska-Rostkowska, Z., Gajewska, J., Zakrzewski, A. J., & Kłębukowska, L. (2023). Prevalence and antibiotic resistance of Bacillus sp. isolated from raw milk. Microorganisms, 11(4), 1065.‏

O'Hara, C. M., Brenner, F. W., & Miller, J. M. (2000). Classification, identification, and clinical significance of Proteus, Providencia, and Morganella. Clinical microbiology reviews, 13(4), 534-546.‏

Stock, I. (2003). Natural antibiotic susceptibility of Proteus spp., with special reference to P. mirabilis and P. penneri strains. Journal of chemotherapy, 15(1), 12-26.‏

Kim, B. N., Kim, N. J., Kim, M. N., Kim, Y. S., Woo, J. H., & Ryu, J. (2003). Bacteraemia due to tribe Proteeae: a review of 132 cases during a decade (1991–2000). Scandinavian journal of infectious diseases, 35(2), 98-103.‏

Owoseni, M. C., Oyigye, O., Sani, B., Lamin, J., & Chere, A. (2021). Antimicrobial resistance and Virulence genes profiling of proteus species from poultry farms in Lafia, Nigeria. BioRxiv, 2021-01.‏

Bush, K., Jacoby, G. A., & Medeiros, A. A. (1995). A functional classification scheme for beta-lactamases and its correlation with molecular structure. Antimicrobial agents and chemotherapy, 39(6), 1211-1233.‏

Perilli, M., Segatore, B., Rosaria De Massis, M., Riccio, M. L., Bianchi, C., Zollo, A., ... & Amicosante, G. (2000). TEM-72, a new extended-spectrum β-lactamase detected in Proteus mirabilis and Morganella morganii in Italy. Antimicrobial agents and chemotherapy, 44(9), 2537-2539.‏

Pagani, L., Migliavacca, R., Pallecchi, L., Matti, C., Giacobone, E., Amicosante, G., ... & Rossolini, G. M. (2002). Emerging extended-spectrum β-lactamases in Proteus mirabilis. Journal of clinical microbiology, 40(4), 1549-1552.‏

Song, W., Kim, J., Bae, I. K., Jeong, S. H., Seo, Y. H., Shin, J. H., ... & Lee, K. (2011). Chromosome-encoded AmpC and CTX-M extended-spectrum β-lactamases in clinical isolates of Proteus mirabilis from Korea. Antimicrobial agents and chemotherapy, 55(4), 1414-1419.‏

Philippon, A., Labia, R., & Jacoby, G. (1989). Extended-spectrum beta-lactamases. Antimicrobial agents and chemotherapy, 33(8), 1131-1136.‏

Stock, I. (2003). Natural antibiotic susceptibility of Proteus spp., with special reference to P. mirabilis and P. penneri strains. Journal of chemotherapy, 15(1), 12-26.‏

Gales, A. C., & Jones, R. N. (2000). Antimicrobial activity and spectrum of the new glycylcycline, GAR-936 tested against 1,203 recent clinical bacterial isolates. Diagnostic microbiology and infectious disease, 36(1), 19-36.‏

Mokracka, J., Gruszczyńska, B., & Kaznowski, A. (2012). Integrons, β-lactamase andqnrgenes in multidrug resistant clinical isolates ofProteus mirabilisandP. vulgaris. APMIS, 120(12), 950-958.‏

Guillard, T., Grillon, A., de Champs, C., Cartier, C., Madoux, J., Berçot, B., ... & Cambau, E. (2014). Mobile insertion cassette elements found in small non-transmissible plasmids in Proteeae may explain qnrD mobilization. PLoS One, 9(2), e87801.‏