Impact of acute stress on macrophage and NK cells in vaccinated Wistar albino rats with recombinant hepatitis-B vaccine

Ashur Lmrabet; Winarto Reki; Edi Dharmana; Dwi Pudjonarko

doi:10.59743/aujas.v4i2.1586

Authors

Ashur Lmrabet Public Health department, Faculty of Medical Technology Msallata, Almergib University
Winarto Reki Faculty of medicine, Diponegoro University
Edi Dharmana Faculty of medicine, Diponegoro University
Dwi Pudjonarko Faculty of medicine, Diponegoro University

DOI:

https://doi.org/10.59743/aujas.v4i2.1586

Keywords:

Natural Killer cells, Macrophage Phagocytosis Index, Acute-stress

Abstract

Background: Hepatitis B virus infection is a global concern health problem. Vaccination with Hepatitis B vaccine is the most efficient way to prevent the infection. Acute Stress may act as an effective adjuvant to increase the immune response to hepatitis B vaccine.

Objectives: To measure the impact of acute-stress on the immune response to hepatitis B vaccinated rats.

Methods: Pretest-Posttest control group design was conducted using animal models (24 Wistar Albino male rats). These were allocated into 4 vaccine groups: one period of acute-stress (X1), two periods of acute-stress (X2) and two groups control (C1, C2). Subsequent the adaption period, rats were given the hepatitis B vaccine.

Results: Data showed that two periods of acute-stress combined with two doses of vaccine led to an increase of macrophage number (%) and macrophage phagocytosis index (%). The macrophage number was (0.51 ± 0.038 in C2 and (0.90 ± 0.025) in X2, (p = 0.001). The macrophage phagocytosis index was (3.42 (3.21 – 3.90) in C2 and (5.75 (5.57 – 6.81) in X2, (p = 0.004). While, NK cells in vaccinated rats showed no response to acute stress.

Conclusion: Acute-stress acts as vaccine adjuvant and has postive effects, particularly on macrophage cells.

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