دراسة قابلية الأيوض الثانوية لبكتيريا .Bacillus spp على تثبيط  تكون الأغشية الحيوية

Eman A A.  Al-Imara; Ghaidaa J.A.  Al-Gazzawy

doi:10.59743/jmset.v5i2.59

Authors

Eman A A. Al-Imara Department of Biotic Evolution, Marine Science Center, Basrah University, Basrah, Iraq.
Ghaidaa J.A. Al-Gazzawy Department of Biology, Collage of Education for Pure Science, Basrah University, Basrah, Iraq.

DOI:

https://doi.org/10.59743/jmset.v5i2.59

Keywords:

Secondary metabolites, Bacillus spp., Antibacterial activity, Biofilms, Antibiotics

Abstract

Twenty bacterial isolates were isolated from different locations in Basrah Governorate, southern Iraq. The isolates were identified biochemically by VITEK2 BCL cards, which showed that these isolates belong to two species of Bacillus. Also, five biofilm-forming bacterial isolates (target bacteria) were isolated and identified by VITEK GP & GN cards, the identification results showed that 3 isolates belong to gram-positive bacteria: Staphylococcus sciuri, Methicillin Resistant Staphylococcus aureus (MRSA), and Kocuria kristinae whereas 2 isolates belong to gram-negative bacteria: Pseudomonas aeruginosa, and Escherichia coli.

The secondary metabolites (SM) were produced, extracted, and purified from Bacillus spp. their antibacterial activity against gram-positive was higher than those against gram-negative bacteria, and results of Thin Layer Chromatography showed that Bacillus secondary metabolites contain free amino acids, this was confirmed by GC-MS analysis, total proteins were determined to choose the isolates which produce the higher quantity of secondary metabolites, so SM extracts of BS8 and BS14 were chosen to complete the study.

Results of quantitative determination of biofilm which formed by target bacteria in microtiter plate showed that P. aeruginosa was the higher biofilm producer among gram-negative bacteria while S. sciuri was the less biofilm producer. Minimum Inhibitory Concentration (MIC) was determined against target bacteria. The inhibitory effect of BS8 and BS14 SM in the inhibition of biofilm formation of P. aeruginosa and S. sciuri in microtiter plate in comparison to the inhibitory effect of nalidixic acid and tetracycline was studied, and the results showed that there was a decrease in the percentage of both bacterial species especially from the MIC of the s. m. extract and the concentrations beyond, the inhibitory effect of the antibiotics was less than that of the SM extracts.

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