Expression of Molt-inhibiting Hormone in Brain and Thoracic Ganglion of Carcinus maenas
DOI:
https://doi.org/10.59743/jaf.v7i2.338Keywords:
MIH, Crustaceans, Ecdysteroid, Thoracic ganglion, Eyestalk ablation, Y-organAbstract
The neuropeptide hormone known as "Molt Inhibiting Hormone (MIH)," which inhibits ecdysteriods by blocking the Y-organ, is generated by the eyestalks of crustaceans. Typically, eyestalk ablation (ESA) can be used to induce molting. ESA does not, however, affect mature a Carcinus maenas. Haemolymph ecdysteroid titers slightly rise after eyestalk ablation., however premolt does not occur right away in animals. While most animals with ES ablation do not molt at all, some do so after many months. As we anticipated, MIH might have been derived from the brain, thoracic ganglion, or other parts of the nervous system. By employing nested endpoint RT-PCR, it is possible to determine the presence of MIH transcript in the brain and thoracic ganglions of intermolt crabs. Sequencing validated the PCR products' identification as MIH. The impact of ESA on MIH expression was investigated using quantitative PCR. Thoracic ganglions and brains were extracted from both the green and red color morphs at 7 and 14 days following the ESA. Unaltered animal tissues served as the reference. The MIH gene's transcriptional regulation remained unchanged following the dissection of the eyestalks, as evidenced by the identical MIH expression between the color morphs and the little influence of ESA on MIH transcript levels. The findings demonstrate that if the primary supply of MIH is eliminated by ESA, brain and/or thoracic ganglion neurons generate enough MIH to prevent molt induction.
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