Amplification of DNA Template Using Polymerase Chain Reaction (PCR) and Using Different Methods in Order PCR Optimization Which Includes Real-Time Quantitative PCR (qPCR)
DOI:
https://doi.org/10.59743/Keywords:
Amplifying, DNA, PCR, Mg concentration, MT temperatureAbstract
Polymerase chain reaction (PCR) is used to amplify a sample of DNA to produce from thousands up to millions of DNA copies and different optimization techniques such as real-time quantitative PCR (q PCR) can be used to optimize the PCR reaction. The results it can be seen that the neat sample had an increased DNA template while contain more than . The optimal temperature for the specific products was 65°C which is the highest annealing temperature and there was no PCR products. However, the low temperature bands of 46°C and the high-temperature ones 58°C had the nearly same brightness as did the bands at 52°C and 55°C. the optimal amplification of the DNA sample occurs with 2.0 and 3.0 mMol concentration of Mg+2 though the alternative Mg+2 concentrations have amplification, but not as specific and the primer dimer gel has nonspecific bands.Downloads
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